產(chǎn)品說明 ：
產(chǎn)品貨號(hào)Reference: CAN-RSN-4000
檢測方法Kit Type: Sandwich ELISA
規(guī)格Kit Size: 96-well break-apart microplate
靈敏度Sensitivity: 0.004 ng/mL
檢測樣本Sample Type: Human Serum / 50 μL
檢測時(shí)間Total Assay Time: 105 minutes
背景介紹：
抵抗素是一種含有108個(gè)氨基酸的12.5 kDa蛋白。它被合成為一種前肽，其疏水性信號(hào)肽在分泌之前被裂解。抵抗素在人的血液中以二聚蛋白的形式循環(huán)，它由兩個(gè)92個(gè)氨基酸的多肽組成，通過一個(gè)二硫鍵連接在一起。
抵抗素屬于類抵抗素激素家族。RELM家族包括RELM-α、RELM-β、RELM-γ和Resistin。RELM-β與抵抗素相關(guān)，在結(jié)腸表達(dá)。在嚙齒動(dòng)物中，抵抗素是由脂肪組織產(chǎn)生的，是葡萄糖代謝和胰島素敏感性的重要調(diào)節(jié)因子。嚙齒動(dòng)物的高抵抗性血癥導(dǎo)致胰島素抵抗和易患2型糖尿病。在人類抵抗素是由巨噬細(xì)胞產(chǎn)生的，它刺激巨噬細(xì)胞分泌促炎癥細(xì)胞因子。一些研究表明血清抵抗素水平升高與動(dòng)脈粥樣硬化有關(guān)。另一項(xiàng)研究顯示，有動(dòng)脈粥樣硬化病變的小鼠體內(nèi)抵抗素水平升高。許多研究試圖通過回答在人類肥胖、胰島素抵抗和/或2型糖尿病中抵抗素水平是否增加的問題來將小鼠數(shù)據(jù)翻譯給人類。一些研究小組未能確定抵抗素水平的變化與肥胖、胰島素抵抗或2型糖尿病的關(guān)系，而另一些使用不同人群和不同檢測方法的研究發(fā)現(xiàn)，抵抗素水平的變化與一種或多種這類疾病有顯著的關(guān)系。
基于上述研究，抵抗素可能是代謝性和炎癥性疾病的生物標(biāo)記物和介質(zhì)。抵抗素生理學(xué)的許多領(lǐng)域還有待于研究，以確定它是否可以作為能量代謝和體重調(diào)節(jié)、代謝綜合征、炎癥和動(dòng)脈粥樣硬化的標(biāo)記物。
Resistin is a 12.5 kDa protein containing 108 amino acids. It is synthesised as a pre-peptide and its hydrophobic signal peptide is cleaved before secretion. Resistin circulates in human blood as a dimeric protein consisting of two 92 amino acid polypeptides that are linked by a disulfide bridge.
Resistin belongs to the resistin-like molecule (RELM) hormone family. The RELM family comprises RELM-α, RELM-β, RELM-γ and resistin. RELM-β is related to resistin and is expressed in the colon. In rodents, resistin is produced by adipose tissue and is a significant regulator of glucose metabolism and insulin sensitivity. Hyperresistinemia in rodents causes insulin resistance and predisposition to type 2 diabetes. In humans, resistin is produced by the macrophages, which stimulates the macrophage secretion of pro-inflammatrory cytokines. Some studies have shown a correlation between increased serum resistin levels and atherosclerosis. Another study shows an increase of resistin levels in mice with atherosclerotic lesions. Many studies have tried to translate the mouse data to humans by answering the question whether levels of resistin are increased in human obesity, insulin resistance, and/or type 2 diabetes. Some groups failed to identify changes in resistin levels with obesity, insulin resistance, or type 2 diabetes while other studies that used diverse populations and different assays, have found significant relationships with one or more of these conditions.
Based on the above studies resistin may be a biomarker and a mediator of metabolic and inflammatory diseases. Many areas of resistin physiology remain to be investigated to determine if it can be used as a marker for energy metabolism and body weight regulation, metabolic syndrome, inflammation and atherosclerosis.
注：本產(chǎn)品僅用于科研用途，不能直接用于人體或臨床診斷使用。
